In KEGG pathway enrichment, terms such as ubiquitin-mediated proteolysis, neurotrophin signaling pathway and cancer-related items such as glioma, hedgehog-signaling pathway and hepatocellular carcinoma were enriched (Supplementary Figure S3D), which indicates that miR-654-5p might be involved in various aspects of carcinogenesis and tumor progression. This evidence concerns the gene BDNF and neoplasm.