In KEGG pathway enrichment, terms such as ubiquitin-mediated proteolysis, neurotrophin signaling pathway and cancer-related items such as glioma, hedgehog-signaling pathway and hepatocellular carcinoma were enriched (Supplementary Figure S3D), which indicates that miR-654-5p might be involved in various aspects of carcinogenesis and tumor progression. The gene discussed is BDNF; the disease is central nervous system cancer.