Compounds 14 (Figure 3) were identified as novel irreversible FAKIs with good potency against FAK (IC50 values ranging from 0.6 nM to 16.3 nM) and able to retard tumor cell growth in GBM cell lines (U-87 MG, U251, and A172 cell lines), strongly reducing U-87 cell migration and cell cycle progression. This evidence concerns the gene PTK2 and neoplasm.