Furthermore, in the cohort reported by Nallamilli and coworkers [9], comprising 4656 United States of America patients studied with an NGS panel of 35 LGMD-causing genes, the diagnostic yield was 27% and CAPN3, DYSF, FKRP and ANO5 were the most affected genes. This evidence concerns the gene ANO5 and limb-girdle muscular dystrophy.