Another study based on 439 patients with NAFLD (78 in an initial and 361 invalidation cohorts) and in controls with cholestatic liver disease (n = 44), found that more advanced NAFLD, as indicated by the presence of NASH with advanced fibrosis stage, is strongly associated with low circulating levels of DHEAS [19]. The gene discussed is SULT2A1; the disease is Cholestatic liver disease.