There were no shared exonic variants in any known mammalian Eph and ephrin genes (EPHA1–EPHA8, EPHA10, EPHB1–EPHB4, EPHB6, EFNA1–EFNA5, and EFNB1–EFNB3) that fulfilled the MAF < 0.01 criteria in all seven spina bifida probands. Here, EPHA8 is linked to spina bifida.