Out of the functional pathways, TGF-beta and VEGF signaling pathways were important; numerous cellular processing pathways include relaxin signaling, cytochrome P450 pathways, apelin signaling, circadian entrainment, chemical carcinogenesis, dilated cardiomyopathy, and axon guidance pathways enriched among bovine granulosa cells in response to acute heat stress. This evidence concerns the gene APLN and dilated cardiomyopathy.