Further, as HS diet is known to induce inflammatory activation of CD4+Tcells, we next determined the inflammatory gene expression (IL1β and IFNγ) in the tumor-infiltrating, lung-infiltrating and circulating CD4+T cells in various salt-diet-modified breast-tumor-bearing mice cohorts isolated by the MACS beads method and with purity ascertained to be >95% by flow cytometry analysis. This evidence concerns the gene IFNG and neoplasm.