Of 15 identifed proteins in the present study, the GO anlaysis showed that almost 40% were related to metabolic/carboxypeptidase activity, which are the keys to pathogenesis of a simple non-alcoholic fatty liver; the GO analysis showed that another 40% were related to immune dysregulation, including CADM1, Prolactin-inducible protein (PIP), and ORM1 and ORM2, of which the immune dysregulation was of liver inflammation and a progression to non-alcoholic steatohepatitis (Ref. Here, PIP is linked to metabolic dysfunction-associated steatohepatitis.