In the present study, all the factors mentioned above deregulated the carbohydrate, glyco-, inflammatory response, insulin, PI3K–Akt signaling pathway, and cholesterol metabolism-related alteration, which could be detected in the urine of MAFLD patients, providing an early and non-invasive insight into the diagnosis of hepatic steatosis. The gene discussed is AKT1; the disease is Hepatic steatosis.