NOTCH1 and B-cell chronic lymphocytic leukemia: Subsequently, another group used ddPCR for assessing NOTCH1 mutations in a larger series of CLL patients: with a LOD of 5 × 10−4, NOTCH1 mutations were detected in 25% of the whole series and in the 55% of patients with trisomy of chromosome 12, with a significant poor prognostic impact [85].