No cytoplasmic MEG3 signal was detected in the U2OS cells expressing MEG3-MS2, demonstrating that MEG3 is a nuclear retained lncRNA, as previously documented in normal fibroblasts such as HFF-1 (human foreskin fibroblast), S27 (Human Foreskin Fibroblast), and W138 (Human Lung Fibroblast) cells as well as a cancerous cell line such as hLF (Human Lung Fibroblast Epidermoid Carcinoma) [37,42]. The gene discussed is MEG3; the disease is squamous cell carcinoma.