Therefore, the future studies will be: (1) to knock out Notch1 and TGF-β genes in cells to test if this knockout can abolish the anti-colon cancer effects of the combination of luteolin and curcumin; (2) chemicals will be dietarily supplemented to xenograft or chemically induced colon cancer mice to test the synergistic anti-colon cancer effects by the combination of luteolin and curcumin; (3) whole-genome RNA-sequencing and metabolomic analysis of tumors will figure out comprehensive mechanisms on how combined luteolin and curcumin synergistically inhibit colon cancer. Here, NOTCH1 is linked to malignant colon neoplasm.