Therefore, the future studies will be: (1) to knock out Notch1 and TGF-β genes in cells to test if this knockout can abolish the anti-colon cancer effects of the combination of luteolin and curcumin; (2) chemicals will be dietarily supplemented to xenograft or chemically induced colon cancer mice to test the synergistic anti-colon cancer effects by the combination of luteolin and curcumin; (3) whole-genome RNA-sequencing and metabolomic analysis of tumors will figure out comprehensive mechanisms on how combined luteolin and curcumin synergistically inhibit colon cancer. This evidence concerns the gene TGFB1 and malignant colon neoplasm.