There are several limitations of this study: (1) if knocking out genes of Notch1 and TGF-β abolish the anti-colon cancer effects of the combination of luteolin and curcumin; (2) IP injection of chemicals is not a typical approach in nutrition research; (3) a comprehensive study of the molecular mechanisms of the anti-colon cancer effects of the combination of luteolin and curcumin is required. This evidence concerns the gene NOTCH1 and colonic neoplasm.