For instance, the combination C15l30 synergistically affected protein levels of Notch1 and TGF-β and the rate of necrosis in tumors, which are in line with its inhibitory effects in cells and tumors, indicating that the combination C15L30 may inhibit colon cancer by regulating Notch1 and TGF-β pathways. This evidence concerns the gene NOTCH1 and colonic neoplasm.