The TMB-H CRC mutations were dispersed over the codons of 516–2620, while the TMB-L CRC contained 58% (11/19) of amino acid changes within the mutation cluster region (MCR, codons 1282–1581), which is in the central part of the APC coding frame involved in β-catenin downregulation [18]. This evidence concerns the gene APC and colorectal carcinoma.