On the AmpliSeq panel, 13 HRR pathway genes are present: ATM, PTEN, BRIP1, ATR, PALB2, MRE11A, RAD50, CHEK1, CHEK2, FANCD2, FANCA, XRCC2 and CDK12. The Figure 4 Oncoplot shows the co-occurrence of multiple mutations in either two of ATM, CDK12, PTEN and ATR in the TMB-H MSS CRC identified here for the first time (Supplementary Table S2). This evidence concerns the gene FANCD2 and colorectal carcinoma.