LncRNA PVT1 was overexpressed in CRC cells and specimens with 5-FU resistance, which might be attributed to the fact that PVT1 increased CRC resistance to 5-FU by inhibiting apoptosis and upregulating the expression of MRP1, P-GP and Bcl-2 mRNA and protein. The gene discussed is PVT1; the disease is colorectal carcinoma.