The resulting method, termed the “PapGene” test, employs the so-called Safe Sequencing System (Safe-SeqS) to achieve reliable detection of mutations from the cells and cellular debris collected via Pap specimens in any of 12 genes (APC, AKT1, BRAF, CTNNB1, EGFR, FBXW7, KRAS, NRAS, PIK3CA, PPP2RIA, PTEN, and TP53) found to be mutated in ovarian and endometrial cancers [192]. This evidence concerns the gene PTEN and endometrial cancer.