In our PDX model, trametinib induced a more evident fold increase in CD103+CD8+ TRM cells in tumor implants of PDX mice that received RepTILs with high CD4+ TIL numbers, and the transfer of RepTILs rich in CD8+ TILs encompassed an increase in intratumoral TRM cells regardless of trametinib. The gene discussed is CD8A; the disease is neoplasm.