IRE increased the percentage of IFN-γ+ CD8+ T cells in splenocytes and increased tumor infiltration of CD8+ T cells. On day 7, reductions of both peripheral and intratumoral Treg cells and PD-1+ T cells were shown. Mice rejected the tumor re-challenge with hepatocellular carcinoma cells following IRE. This evidence concerns the gene IFNG and hepatocellular carcinoma.