EPCAM and neoplasm: Confirming the standard IF×3 protocol, we observed that CTCs in each of the above-mentioned samples were more than 15–20 micron in size with an evident pathological/morphological nuclear characteristic of a tumor cell (a nuclear/cytosol ratio > 50%) by DAPI and were CK8,18,19+/EpCAM+/CD45−/DAPI+ when stained using the DAPI/CK-FITC/EpCAM-PE/CD45-Cy5 antibodies.