Since we did not have the confocal images, we could identify the plasma-membrane EpCAM positivity of a tumor cell depending on the orientation of the cell on the microfilter as shown in HCC1975 (Figure 1C) and NCI-H441 (Figure 1D) cells using the DAPI/CK-FITC/EpCAM-PE/CD45-Cy5 antibodies. This evidence concerns the gene EPCAM and neoplasm.