To test this possibility, the lactate released to the media by cells with low-CAV1 levels (mouse melanoma B16F10(Mock), human breast cancer MDA-MB-231(shCAV1), and human colon adenocarcinoma HT29(US)(Mock)) was compared with the lactate levels in media from cells with high CAV1 expression (B16F10(CAV1), MDA-MB-231(shC), and HT29(US)(CAV1)). Here, CAV1 is linked to melanoma.