To elucidate a novel function of p-PDHA1 in HepG2 hepatocellular carcinoma cells, which are sensitive to insulin signaling, we identified the binding protein that may interact with p-PDHA1 using a recombinant GST phospho-mimic mutant PDHA1 Ser293Glu (S293D). The gene discussed is PDHA1; the disease is hepatocellular carcinoma.