Despite the lack of replication of lower DNA methylation levels at the ESM1, TPSAN14, and MS4A3 DNA regions, as previously observed in GDM exposed offspring at childhood age, in this study, we observed differential transcriptional changes of ESM1 and TSPAN14 in the adipose tissue of adult offspring exposed to GDM in pregnancy compared to non-exposed offspring. The gene discussed is MS4A3; the disease is gestational diabetes.