NAC treatment in T2D mice effectively preserved the expression of the antioxidant enzymes (SOD-1, Gpx-1, and catalase), while attenuating ROS production in the diabetic ischemic limb together with reversed levels of phosphorylated IRS-1, Akt, and eNOS, and plasma TNF-a. This evidence concerns the gene AKT1 and type 2 diabetes mellitus.