In this NAFLD model, the increased expression of ER stress-related genes, including Chop, Grp78, Atf6b, Ire1, Xbp1, Traf3, Hyou1, Syvn1, and Gm3776, indicates the induction of ER stress in NAFLD. This evidence concerns the gene TRAF3 and metabolic dysfunction-associated steatotic liver disease.