In this regard, we established a cohort of APP-KINL-G-F and APP-KINL-NL animals of 2 different age groups: 3- and 6-months of age (refer to Methods for specific animal cohorts and numbers), and injected them with either AD-tau seeding material or buffer solution (i.e., phosphate-buffered saline) into the hippocampus, resulting in a total of 4 genotype-treatment animal groups: NL-G-F animals injected with tau seeds (NL-G-F-tau), NL-G-F animals injected with buffer solution (NL-G-F-buffer), NL/NL animals seeded with tau (NL-tau) and NL/NL animals injected with buffer solution (NL-buffer). The gene discussed is MAPT; the disease is Alzheimer disease.