As the adiposity-reducing properties of RYGB microbiota transfer appear to be clearly dependent on intact gut FXR signaling, but only partly attributable to suppressed appetite (Supplementary Figures S5 and 10c), we further investigated the role of intestinal FXR signaling in accelerated metabolic rate and increased adipose tissue thermogenesis following RYGB microbiota transfer to HF-DIO recipients. This evidence concerns the gene NR1H4 and hydrops fetalis.