Of the 268 participants who did not have a pre-existing diagnosis of depression and anxiety, 13% (n = 36) had a positive PRIUSS score, of which 81% (n = 29) had a positive GAD-7 screen (7 participants with mild anxiety, 9 participants with moderate anxiety and 13 participants with severe anxiety), 83% (n = 30) had a positive PHQ-9A screen (12 participants with mild depression, 10 participants with moderate depression, 4 participants with moderately severe depression and 4 participants with severe depression) and 75% (n = 27) had positive GAD-7 and PHQ-9A screens. This evidence concerns the gene GAD1 and major depressive disorder.