To confirm that oxalate accumulation induced by primary cancer was HAO1 dependent, we performed a once daily injection of CCPST after orthotopic implantation of 4T1 cells in the fat pad, or injection of sgHao1 AAV via the tail vein the day before orthotopic implantation of 4T1 cells in the fat pad, and then detected the pulmonary oxalate concentration after two weeks. Here, HAO1 is linked to cancer.