TNF and neoplasm: We also examined the content of some cytokines and chemokine in tumor that are crucial for antitumor immunity within TME via the employment of ELISA assay as previously described.46 It is revealed that the contents of TNF-α, IL-6 and IFN-γ were prominently increased after the treatment with anti-PD-1 antibody, and further potentiated after the combination with miR-21–3 p-AuNp.