Subsequent firefly luciferase reporter assay showed that agomiR-21–3p significantly suppressed the luciferase activity in melanoma cells transfected with a plasmid harboring WT TXNRD1 3’UTR, but failed to influence the reporter activity in cells transfected with plasmids harboring mutant TXNRD1 3’UTR (figure 3B). Here, TXNRD1 is linked to melanoma.