ESR1 and Cushing syndrome due to macronodular adrenal hyperplasia: When comparing the canonical pathways significantly affected in the present study and in humans with atherosclerosis, most were predicted to have contrary activation patterns (Figure 6): the canonical pathways phospholipase C signaling, integrin signaling, IL8 signaling, CXCR4 signaling, estrogen receptor signaling, GNRH signaling, signaling by Rho Family GTPases, HIF1α signaling, thrombin signaling and relaxin signaling were predicted to be deactivated in the VAT of dogs with Cushing’s syndrome but activated in humans with atherosclerosis.