Analysis of IFN-γ gene signature of 18 genes (T-cell-inflamed gene expression profile, TcellinfGEP) in HNSCC patient tissues from KEYNOTE-012 study showed independent predictive values of TMB and GEP score with ORR to pembrolizumab therapy response in HPV and EBV negative HNSCC. This evidence concerns the gene IFNG and head and neck squamous cell carcinoma.