However, in a study of triple-negative breast cancer, transfection of MCF-7 and MDA-MB-231 cells to overexpress or silence TMEM88 did not affect the activity of the canonical Wnt signaling pathway or the expression of corresponding target genes such as MMP-7, c-Myc and cyclin D1 (30). The gene discussed is CCND1; the disease is triple-negative breast carcinoma.