One study administered L. rhamnosus GG at 1.8 × 1010 CFU/day on pregnant women with a high allergic disease risk from 36 weeks of gestation to delivery (n = 73) (44) and found a lower level of total IgA seen in the 28-day mature milk as compared to the placebo group but no significant differences were seen in total IgA in the 7-day breast milk samples and no significant difference in breast milk TGF-β1 compared with the placebo group at 7 or 28 days. This evidence concerns the gene TGFB1 and allergic disease.