CD8A and infection: To further analyze the multidimensional data of our multi-parametric analysis, and to look for a potential molecular pattern of virus-specific CD8+ T cells at different disease stages using unbiased approaches, we performed tSNE analysis looking at patients with acute HCV infection [aHCV (black)]; cHCV infection and on-target T cells [on-target (red)]; cHCV infection and off-target T cells [off-target (purple)]; resolved infection [rHCV (blue)] and successfully treated patients [SVR (green)] (Figure 2A) (50).