Hallmark enrichment showed that subcluster 3 were specially enriched in MYC-TARGET_V1 pathway (Fig. 3G, Table S5), and representative gene clusters such as AP3S1, BUB3, EIFD3, LDHA, NMP1, PSMD14, SERBP1, SSBP1, and UBE2L3 affected overall survival based on the TCGA-PAAD data (Fig. 3H and I, Table S5). The gene discussed is BUB3; the disease is pancreatic adenocarcinoma.