To explore the mechanism of lncRNA XIST in PCOS, KGN cells were transfected with a control-plasmid or an XIST-plasmid, a mimic control or a miR-30c-5p mimic, an XIST-plasmid+mimic control or an XIST-plasmid+miR-30c-5p mimic to determine whether XIST regulates miR-30c-5p expression in granulosa cells. Here, XIST is linked to polycystic ovary syndrome.