To understand how BCYRN1 impacts HCC cell characteristics, HCCLM3 cells were next transfected with OV-NC + sh-NC, OV-BCYRN1 + sh-NC, OV-BCYRN1 + sh-BATF, or OV-BCYRN1 + sh-TM4SF1, and their proliferative and invasive activities were then assessed. The gene discussed is TM4SF1; the disease is hepatocellular carcinoma.