Our data suggest that pretreatment with Rg1 and ADSC + Rg1 markedly decreases the levels of TLR4 and MyD88, as well as the expression of TNF-α, IL-6, IL-1β, IFN-γ, and IL-17A in the DSS-induced mouse model of colitis. This evidence concerns the gene TLR4 and colitis.