With this procedure, we recorded about 100 STED platelet images each, for six different proteins (VAMP7, VAMP8, STX11, SNAP23, TSP1, and VEGF) and for five different categories of platelets; incubated with cancer cells (MCF-7, MDA-MD-231, EFO-21), non-cancer cells (MCF-10A), or no cells at all (R). Here, THBS1 is linked to cancer.