Multiplex immunostaining of bladder tumor specimens from 400 patients revealed that a high level of TFCP2L1 and CDK1 coexpression is associated with unfavorable clinical characteristics, including high tumor grade, lymphovascular invasion, muscularis propria invasion, and distant metastasis, and is also an independent prognostic factor for cancer-specific survival. This evidence concerns the gene TFCP2L1 and urinary bladder neoplasm.