GHR and liver cancer: To validate the specificity and selectivity of isomiR-21-5p | +1 and isomiR-21-5p | -1 targeting GHR, we constructed two different psiCHECK-2-GHR-3′-UTR vectors containing either the isomiR-21-5p | +1 site (GHR-3′-UTR#1) or both isomiR-21-5p | ±1 sites (GHR-3′-UTR#2) downstream of a luciferase reporter gene (Fig. 7c) and transformed them into liver cancer cells.