Non-alcoholic steatohepatitis (NASH) is the most severe form of non-alcoholic fatty liver disease (NAFLD) and supposed as a potential precursor of HCC.28 16 weeks after HFD feeding, fasting serum glucose and insulin levels were significantly decreased in Mat2a LKO mice (Supplementary Fig. S3d, e). Here, INS is linked to hepatocellular carcinoma.