INS and metabolic dysfunction-associated steatohepatitis: Non-alcoholic steatohepatitis (NASH) is the most severe form of non-alcoholic fatty liver disease (NAFLD) and supposed as a potential precursor of HCC.28 16 weeks after HFD feeding, fasting serum glucose and insulin levels were significantly decreased in Mat2a LKO mice (Supplementary Fig. S3d, e).