Non-alcoholic steatohepatitis (NASH) is the most severe form of non-alcoholic fatty liver disease (NAFLD) and supposed as a potential precursor of HCC.28 16 weeks after HFD feeding, fasting serum glucose and insulin levels were significantly decreased in Mat2a LKO mice (Supplementary Fig. S3d, e). This evidence concerns the gene MAT2A and metabolic dysfunction-associated steatotic liver disease.