Interestingly, if mice were neutrophil depleted by using a depleting antibody (clone RB6-8C5, 30 mg/mouse i.p. 24 hours before the experiment) and reconstituted with donor neutrophils isolated form the BM of WT mice, incubation of the donor neutrophils with the TLR4 inhibitor TAK-242 prior to injection into the recipient mice protected the recipient from AKI elicited by subsequent IRI surgery compared with mice that were reconstituted with vehicle-treated neutrophils (Supplemental Figure 2, H and I). The gene discussed is TLR4; the disease is acute kidney injury.