CH223191 pretreated mice had aggravated endometritis compared with E. coli treatment, as shown by increased macroscopic inflammatory changes (Fig. S1A in the supplemental material), barrier disruption and inflammatory infiltrations (Fig. S1B), histological score (Fig. S1C), MPO activity (Fig. S1D), inflammatory cytokines (Fig. S1E and F), and reduced occludin and claudin-3 levels (Fig. S1G–I) compared with E. coli-treated mice. This evidence concerns the gene CLDN3 and endometritis.