Second, GO and KEGG pathway enrichment analysis showed that 20 upregulated genes enriched in endodermal cell differentiation, protein heterotrimerization, ECM-receptor interaction, focal adhesion, protein digestion and absorption, PI3K-Akt signaling pathway, amoebiasis, and platelet activation, while 49 downregulated genes enriched in digestion, potassium ion import, oxidation-reduction process, bicarbonate transport, inward rectifier potassium channel activity, hydrogen:potassium-exchanging ATPase activity, gastric acid secretion, retinol metabolism, and metabolic pathways (P < 0.05). The gene discussed is AKT1; the disease is amebiasis.