Conversely, overexpression of ANKFN1 regulated the ERK/c-Myc/cyclin D1/Cdk4/6 pathway (Figs. 6F, S2) and promoted HCC proliferation, metastasis, invasion and repressed apoptosis in vitro and in vivo (Figs. 5, 6A–E). Here, CDK4 is linked to hepatocellular carcinoma.