Knockdown of ANKFN1 inhibited HCC proliferation and promoted apoptosis in vitro and in vivo (Figs. 2, 3, 4A–F), and we found that p-ERK, c-Myc, cyclin D1, Cdk4/6 and PCNA were downregulated in sh-ANKFN1#3-transfected cells (Fig. 4G). This evidence concerns the gene CDK4 and hepatocellular carcinoma.