In the current study, we discovered that downregulation of miR-486-3p or overexpression of eIF5A restored cell proliferation and invasion after circ-EPB41 silencing, suggesting that circ-EPB41 expression predicts unfavorable prognoses in NSCLC by regulating the miR-486-3p/eIF5A axis. This evidence concerns the gene EPB41 and non-small cell lung carcinoma.