Downregulation of miR-486-3p or overexpression of eIF5A restored CSC differentiation after circ-EPB41 silencing, which suggested that circ-EPB41 expression predicts unfavorable prognoses in NSCLC by regulating miR-486-3p/eIF5A axis-mediated stemness. Here, EPB41 is linked to non-small cell lung carcinoma.