To validate the RNA-seq results, we selected six upregulated circRNAs (hsa_circ_0001721 [circ-CDK14], hsa_circ_0000130 [SNX27], hsa_circ_0001747 [circ-MKLN1], hsa_circ_0001869 [circ-ZCCHC6], hsa_circ_0008992 [circ-PAK1] and hsa_circ_0000042 [circ-EPB41]) for RT-qPCR analysis using ten paired NSCLC tissues. The gene discussed is EPB41; the disease is non-small cell lung carcinoma.