In this study, we combined the anti-PD-L1 scFv-encoding gene to the Neo2/15-encoding gene and expressed a recombinant Neo2/15-conjugated anti-PD-L1 scFv, which can be used for effective immunotherapy as an immunocytokine that has the potential to localize on the surface of tumor cells and to activate anticancer immunity. Here, CD274 is linked to neoplasm.