In this study, we performed a functional and molecular characterization of human gastric CSCs, and demonstrated the cancer stemness inhibition by 4-BR by using different functional approaches and stem-cell-related markers characterization, including stem cell surface markers, CD24 and LGR5; and stemness-related transcription factors, SOX2, NANOG, Oct-4, and detoxifying enzyme, ALDH1. This evidence concerns the gene SOX2 and cancer.