To further explain the regulatory network and molecular mechanisms of CAVD, we filtered 10 hub genes (SPP1, TREM1, GPM6A, CCL19, CR1, NCAM1, CNTN1, TLR8, SDC1, and COL6A6), constructed the LncRNA-miRNA-mRNA network, and calculated the correlation between hub genes and immune cell infiltration, which has not been reported previously. Here, SDC1 is linked to congenital bilateral aplasia of vas deferens from CFTR mutation.