Based on the results of ROC, 10 genes (CR1, TREM1, TLR8, SDC1, CCL19, NCAM1, COL6A6, SPP1, CNTN1, and GPM6A) with AUC > 0.7 were considered the hub genes associated with CAVD. The gene discussed is COL6A6; the disease is congenital bilateral aplasia of vas deferens from CFTR mutation.