We also detected liver FXR, CYP7A1 and TRPV1 (Fig. 5D–F), and found CYP7A1 increased in HFM (by 80.9%), TRPV1 increased in HFM (by 179.7%), and the increased CYP7A1 activity may result in decreased intrahepatic cholesterol concentration (41, 42). This evidence concerns the gene NR1H4 and craniofacial microsomia.