NHRI-1-E4 upregulates expression of distinct genes, e.g., Bmi1, Sox2, Notch1, compared to NHRI-8-B4, suggesting that NHRI-1-E4 relies on Hedgehog, NF-κB or Notch signaling pathways rather than Wnt/β-catenin to maintain its cancer stemness (Yang et al., 2020); these results also imply that the Wnt/β-catenin pathway is the major pathway regulating cancer stemness when aberrant lipid metabolism affects CSC differentiation. This evidence concerns the gene SOX2 and cancer.