In comparison to Hepa1-6, NHRI-1-E4 and NHRI-8-B4 tumor cells also up-regulated the gene expression of CD56 and epithelial cell adhesion molecule (EpCAM) (data not shown), and therefore we classified them as poorly differentiated HCC type with higher proliferative capability and worse prognosis, which can be a feature of PI3K/AKT-mTOR/RAS-MAPK signaling (Giraud et al., 2021). Here, MTOR is linked to hepatocellular carcinoma.